2-day Hands-on HPLC Theory, Methods & Maintenance

Request course outlineBook this courseView dates and bookArrange course onsiteTailor this courseConsultancy on LCHands-on HPLC Theory, Methods & Maintenance

Course summary

  • Number of days: 2
  • Experience level: Intermediate
  • Type: Generic, applicable to all instrumentation
  • Teaching method: 50% face-to-face classroom and 50% practical exercises in the laboratory
  • Course venue: The Open University, Milton Keynes, UK | See individual dates for other venues
  • Onsite/offsite availability: Arrange this course just for you
  • Scheduled course price: £1,075 + VAT per delegate

What will you learn on this course?

Spend the first day learning the theory of liquid chromatography and then using this knowledge to create isocratic and gradient methods, program detectors and analyse data.

The final day combines learning the reasons why maintenance is required, what happens if maintenance isn’t performed and the signs of when maintenance is needed along with practicals carrying out maintenance on a liquid chromatograph.

This course will cover the theory, methods and maintenance of the solvents & buffers (the mobile phase), pumps, isocratic and gradient elution, autosampler, injector, analytical columns, HPLC detectors and data analysis.

Learning outcomes

  • You will learn the importance of good chromatography
  • You will learn about the function & operation of the individual HPLC components
  • You will become familiar with the HPLC instrumentation
  • You will learn the importance of routine maintenance
  • You will learn the signs of when maintenance is required
  • You will have practical experience of adjusting the parameters for each HPLC component
  • You will have practical experience of analysing data
  • You will have practical experience of performing maintenance on each HPLC components

Who is this course for?

This course is suitable for all levels of knowledge; however for complete beginners it is advisable to attend the 1-day Absolute Basics of HPLC & LC-MS first to get a good basic overview of liquid chromatography.

Course combinations

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