Fractions of the effluent from one LC column are collected and re-injected onto a second, faster column containing a different stationary phase. The separation on the second column is completed before the next fraction is transferred. This provides a huge increase in column efficiency and resolving power of the method. Both columns are in tandem and are served by a single detector, but a separate pumping system is required for each column.

LCxLC Training Courses

Classroom-based training courses: